大家在查：表达文库体外转录原核表达分子反应核酸探针技术噬斑全信息毒力因子随机引物法缺口平移

阳性克隆

网络AD-Y

阳性克隆

阳性克隆

阳性克隆

AD-Y

...合菌,根据报告基因的激活有否,筛选出相互作用蛋白的阳性克隆(AD-Y),验证分离单克隆后,抽提质粒,DNA测序,通过生物信息学 …

1

Objective To develope a reliable method for rapidly screening positive clones of recombinant genes.

目的建立一个简便、可靠的重组阳性克隆的筛选方法。

2

Aim : To develop a PCR technique for rapid screening of recombinant plasmid in subtractive library of cDNA .

目的：消减文库构建过程中，用 PCR 技术快速筛选重组阳性克隆。

4

In the forward cDNA libraries, we picked 769 positive clones randomly , which was testified by PCR analysis.

在正向文库中，随机挑取了 769 个阳性克隆，并进行 PCR 验证。

7

Screen for recombinant clones by colony Polymerase chain reaction

应用以菌落为模板的聚合酶链反应技术筛选重组阳性克隆

8

Screening of LoxP-positive recombinant clones by self-primer colony polymerase chain reaction

自身引物菌落聚合酶链反应筛选 LoxP 序列重组阳性克隆

9

Rapid screening E. Coil positive clone by microwave cook

利用微波炉快速筛选大肠杆菌阳性克隆

10

Improving method of selective positive clone by PCR

PCR 筛选阳性克隆方法的改进

热词推荐

大家在看